Fig. 2: DLK regulates axotomy-induced mitochondrial fission.
From: DLK-dependent axonal mitochondrial fission drives degeneration after axotomy
a Representative images of DLK colocalization at the site of mitochondrial fission after axotomy. DLK-GFP (green) and mitochondria (mitoRFP, magenta). Scalebar = 10 µm. b Percentage mitochondrial fission events where DLK is localized at the site of fission. N = 3 independent differentiations, 74 fission events from 33 axotomized neurons. c Representative images of WT and DLK KO neurons transduced with mitoGFP (green) pre (Pre) and 5 min post axotomy (PA). * Marks the site of axotomy. Scalebar = 25 µm. d Normalized number of mitochondrial particles post axotomy in WT (black) and DLK KO (green) neurons. N = 3 independent differentiations, N = 29 WT and N = 30 DLK KO axotomized neurons (Two-way ANOVA, Bonferroni correction (p < 0.0001 ****). e Normalized number of mitochondrial particles post axotomy in DMSO (black) and GNE3511 (green)-treated neurons. N = 3 independent differentiations, N = 23 DMSO and N = 25 GNE3511 axotomized neurons. (Two-way ANOVA, Bonferroni correction, p < 0.0001 ****). f Normalized number of mitochondrial particles post axotomy in DLK KO neurons transduced with WT DLK-GFP (green), DLK-C127S-GFP (black) or DLK-S302A-GFP (purple). N = 3 independent differentiations, N = 31 DLK WT and N = 27 DLK-C127S and N = 23 DLK-S302A axotomized neurons. Two-way ANOVA, Bonferroni correction (p < 0.0001 ****, WT DLK vs DLK C127S, p < 0.0001 ####, WT DLK vs S302A). g Representative images of neurons transduced with mitoGFP (green) treated with DMSO and JNKi pre (Pre) and 5 min post axotomy (PA). * Marks the site of axotomy. Scalebar = 25 µm. h Normalized number of mitochondrial particles post axotomy in DMSO (black) and JNKi (green)-treated neurons. N = 3 independent differentiations, N = 32 DMSO and N = 31 JNKi axotomized neurons. (Two-way ANOVA, Bonferroni correction, p < 0.0001 ****). Graphical representations show mean ± SEM.
