Fig. 7: BAX regulates cell death after axotomy.

a Representative images of WT neurons treated with DMSO or GNE3511 pre- and 1 h post-axotomy (PA) expressing mitoGFP (green) and A₁-BAX/B₁-DRP1 complexes (red). Scale bar = 5 µm. b Quantification of A₁-BAX/B₁-DRP1 fluorescence fold change in the mitochondria of DMSO or GNE3511 treated neurons 1-h post-axotomy. N = 3 independent differentiations, N = 35 DMSO and N = 34 GNE3511 axotomized neurons. Two-way ANOVA, Bonferroni correction. (p = 0.0168 *). c Quantification of A₁-BAX/B₁- WT DRP1 and A₁-BAX/B₁- DRP1 S616A fluorescence fold change in the mitochondria 1-h post-axotomy. N = 3 independent differentiations, N = 37 DRP1 WT and N = 38 DRP1 S616 axotomized neurons. Two-way ANOVA, Bonferroni correction. ( = 0.0192 *). d Representative images of DMSO and BAXi treated neuron cell bodies transduced with cyto mApple (red) pre and 18 h post axotomy (PA). Scalebar 40 µm. e Percent degenerated neurons 18 h post axotomy treated with either DMSO or BAXi. N = 3 independent differentiations. Unpaired, two-tailed, t test. (p = 0.0118 *). f Quantification of axon degeneration index (ADI) of DMSO and BAX-A-treated neurons 24 h after axotomy. N = 3 independent differentiations, total of 46 axons. One-way ANOVA, Bonferroni correction (p ≤ 0.0001 ****, not significant, ns). g Illustration of microfluidic devices used to separate axons from somas allowing for the local treatment of i3Neuron axons. h Representative images of WT axons separated using microfluidic chambers treated with DMSO, BAX-A or BAX-A + GNE3511 for 24 h. Scalebar 25 µm. i Quantification of axon degeneration index (ADI) of isolated axons treated with DMSO BAX-A and BAX-A + GNE3511 for 24 h. N = 3 independent differentiations, 10 images per differentiation. One-way ANOVA, Bonferroni correction (p ≤ 0.0001 ****, not significant, ns). All graphical representations show mean ± SEM.