Fig. 2: Peroxiredoxin 2 facilitates ROS clearance to promote food digestion.
From: Neuronal PRDX-2-Mediated ROS Signaling Regulates Food Digestion via peripheral UPRmt Activation
a Representative microscope images and quantitative analysis of MitoTrackerTM Red CMXRos fluorescent showing increased ROS level in WT and prdx-2(gk169) after being grown on E. coli OP50 for 48 h at 20°C. Obtained p value was: WT vs prdx-2(gk169); p = 0.0009. See more detail method in the methods section. b Representative microscope images and quantitative analysis of col-19p::mito::cpYFP fluorescent in WT animals treated with vector control and prdx-2 RNAi bacteria. The col-19p::mito::cpYFP has been used as a sensor for Mitochondrial ROS46. Obtained p value was: Control RNAi vs prdx-2 RNAi; p < 0.0001. c Developmental progression of synchronized WT, prdx-2(gk169) L1 animals grown on HK-E. coli + SS bacteria for 96 h at 20 °C. Obtained p values (from L4 stage) was: WT vs prdx-2(gk169); p = 0.03. d Developmental progression of synchronized WT, prdx-2(gk169) L1 animals grown on HK-E. coli + SS bacteria supplemented with 50 mM NAC for 96 h at 20°C. Obtained p values (from L4 stage) were as follows: WT vs prdx-2(gk169); p < 0.0001. WT + NAC vs prdx-2(gk169)+NAC; p = 0.29. For all panels, n = the number of worms. Data are represented as mean ± SEM. All statistical analyses were preformed using unpaired two-tailed Student’s t-test.**p < 0.01, ***p < 0.001, n.s., not significant. All experiments were performed independently at least three times. Source data are provided as a Source Data file.
