Fig. 3: prdx-2 knock-out in AWC neuron shut down food digestion.
From: Neuronal PRDX-2-Mediated ROS Signaling Regulates Food Digestion via peripheral UPRmt Activation
a Confocal image of expression pattern of PRDX-2. b–e Developmental progression of prdx-2(gk169) carries with (b) prdx-2p::prdx-2::gfp (by its own promoter expression) or (c) rgef-1p::prdx-2::gfp (neuron-specific expression) or (d) vha-6p::prdx-2::gfp (intestine-specific expression) or (e) odr-1p::prdx-2::gfp (AWC neuron-specific expression) animals quantified by relative worm length. Animals carrying transgenes are labeled in yellow. Obtained p values were as follows: (b) Control vs Transgene; p < 0.0001. (c) Control vs Transgene; p < 0.0001. (d) Control vs Transgene; p = 0.14. (e) Control vs Transgene; p = 0.0001. f Representative microscope images and quantitative analysis of MitoTrackerTM Red CMXRos in WT, prdx-2(gk169) or AWC neuron specific knockout prdx-2 animals which grown on E. coli OP50 for 48 h at 20°C. Obtained p values were as follows: WT vs prdx-2(gk169); p < 0.0001. WT vs AWC prdx-2 KO (ylf40); p < 0.0001. See more detail method in the methods section. g Developmental progression of synchronized WT, prdx-2(gk169) or AWC neuron specific knockout prdx-2 animals L1 grown on HK-E. coli + SS bacteria for 96 h at 20°C. Obtained p values (from L4 stage) were as follows: WT vs prdx-2(gk169); p = 0.0004. WT vs AWC prdx-2 KO (ylf40); p = 0.0003. For all panels, n = the number of worms. Data are represented as mean ± SEM. All statistical analyses were preformed using unpaired two-tailed Student’s t-test. **p < 0.01, ***p < 0.001, n.s., not significant. All experiments were performed independently at least three times. Source data are provided as a Source Data file. See also Supplementary Fig. 3.
