Fig. 4: AWC-specific knockout prdx-2 activates intestinal UPRmt.
From: Neuronal PRDX-2-Mediated ROS Signaling Regulates Food Digestion via peripheral UPRmt Activation
a Representative microscope images and quantitative analysis of hsp-6p::GFP expression in WT and prdx-2(gk169) animals. Obtained p value was: WT vs prdx-2(gk169); p < 0.0001. b Representative microscope images and quantitative analysis of dve-1p::DVE-1::GFP in WT and prdx-2(gk169) animals. Obtained p value was: WT vs prdx-2(gk169); p < 0.0001. c Representative microscope images and quantitative analysis of hsp-6p::GFP expression in WT, prdx-2(gk169), prdx-2(gk169) carries with rgef-1p::prdx-2::mKate2 or odr-1p::prdx-2:: mKate2 animals. Obtained p values were as follows: WT vs prdx-2(gk169); p < 0.0001. prdx-2(gk169) vs ylfEx180 [prdx-2(gk169); rgef-1p::prdx-2::mKate2]; p < 0.0001. prdx-2(gk169) vs ylfEx162 [prdx-2(gk169); odr-1p::prdx-2::mKate2]; p < 0.0001. d Representative microscope images and quantitative analysis of hsp-6p::GFP expression in WT, prdx-2(gk169) or AWC neuron specific knockout prdx-2 animals. Obtained p values were as follows: WT vs prdx-2(gk169); p < 0.0001. WT vs AWC prdx-2 KO (ylf40); p < 0.0001. e Developmental progression of synchronized WT, prdx-2(gk169), atfs-1(tm4525), prdx-2(gk169);atfs-1(tm4525) L1 animals grown on HK-E. coli + SS bacteria for 96 h at 20°C. Obtained p values (from L4 stage) were as follows: WT vs prdx-2(gk169); p < 0.0001. WT vs atfs-1(tm4525); p = 0.03. prdx-2(gk169) vs prdx-2(gk169);atfs-1(tm4525); p = 0.004. For all panels, n = the number of worms. Data are represented as mean ± SEM. All statistical analyses were preformed using unpaired two-tailed Student’s t-test. ***p < 0.001, n.s., not significant. All experiments were performed independently at least three times. Source data are provided as a Source Data file. See also Supplementary Figs. 4–6.
