Fig. 5: Neuropeptide NLP-1 is required for prdx-2-knockout-induced UPR^mt.

a Confocal image of expression pattern of nlp-1. b qRT-PCR analyses of nlp-1 mRNA level in the WT or prdx-2(gk169) animals feeding OP50 or HK-E. coli + SS. Obtained p values were as follows: WT OP50 vs prdx-2(gk169) OP50; p = 0.0003. WT HK-E. coli + SS vs prdx-2(gk169) HK-E. coli + SS; p = 0.0334. c Representative microscope images and quantitative analysis of hsp-6p::GFP expression in WT, prdx-2(gk169), nlp-1(ok1469), prdx-2(gk169);nlp-1(ok1469) animals. Obtained p values were as follows: WT vs prdx-2(gk169); p < 0.0001. prdx-2(gk169) vs prdx-2(gk169);nlp-1(ok1469); p = 0.0372. d Representative microscope images and quantitative analysis of hsp-6p::GFP expression in WT, WT carries with nlp-1p:: nlp-1::3xFlag, odr-1p:: nlp-1::3xFlag and vha-6p::nlp-1::3xFlag animals. Obtained p values were as follows: WT vs ylfEx216[nlp-1p::nlp-1::3xFlag]; p < 0.0001. ylfEx217[odr-1p::nlp-1:: 3xFlag]; p = 0.001. ylfEx218[vha-6p::nlp-1:: 3xFlag]; p < 0.0001. e, f Developmental progression of synchronized WT animals carries with nlp-1p:: nlp-1::3xflag (e) or odr-1p:: nlp-1::3xflag (f) L1 animals, which was quantified as relative worm length. Transgene worms are indicated by a yellow asterisk. Obtained p values were as follows: (e): Control vs Transgene; p < 0.0001. (f): Control vs Transgene; p < 0.0001. For the panels (c–f) n = the number of worms. For panels (b), n = the number of qPCR experiments performed. Data are represented as mean ± SEM. All statistical analyses were preformed using unpaired two-tailed Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001, n.s., not significant. All experiments were performed independently at least three times. Source data are provided as a Source Data file. See also Supplementary Fig. 7.