Fig. 2: Autoclaved vegetables with anisotropic topology induced alignment of C2C12 myoblasts.
From: Growing meat on autoclaved vegetables with biomimetic stiffness and micro-patterns
a (i), b (i) Representative Live and Dead images (n = three independent experiments) of C2C12 cultured on vegetable slices for 2 days. Vegetables were autoclaved, rinsed with deionized water, and soaked in growth medium containing 1% w/v gelatin overnight. Scale bar = 100 μm. a (ii), b (ii)) Distribution of cell alignment angles on respective vegetable slices. The radial axis shows the radial sum intensity and the circumferential axis shows cell orientation angles (0−180 degrees). Panel a (i) and b (i) were, in part, created with BioRender. Lab, D. (2024) https://BioRender.com/x24q862 and with ixintu.com.
