Fig. 3: DDX18 interacts with nucleolar RNAs and GC component proteins. | Nature Communications

Fig. 3: DDX18 interacts with nucleolar RNAs and GC component proteins.

From: DDX18 coordinates nucleolus phase separation and nuclear organization to control the pluripotency of human embryonic stem cells

Fig. 3

a Summary of the DDX18 interactome showing representative GO terms and corresponding proteins. Nucleolar GC marker proteins NPM1 and NCL are also shown under rRNA processing. b RNA-dependent interactions between DDX18 and NPM1. Western blotting detection of DDX18 and NPM1 protein levels after Flag-IP with and without RNase A treatment. IgG serves as the negative control IP. The experiment was repeated independently twice with similar results. c Representative live imaging of reconstituted BiFC fluorescence. Green fluorescence shows the reconstitution of YFP as an indicator of the protein-protein interaction between DDX18 and NPM1. The experiment was repeated independently twice with similar results. d Relative enrichment of different RNA species identified from DDX18 iCLIP-seq. e HOMER motif analysis of DDX18 iCLIP-seq showing the top consensus sequences. The conserved motif of the C/D box snoRNAs is shown at the top. P value was calculated by HOMER. f GO analysis of biological processes for DDX18 bound mRNAs. Source data are provided as a Source Data file.

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