Fig. 2: Lectin tracer levels increase at cell junctions with increasing IOP.
From: FYN regulates aqueous humor outflow and IOP through the phosphorylation of VE-CADHERIN
A. Experimental scheme, B. Prox1-GFP eyes perfused with lectin at 16, 25 or 45 mmHg. Top panels, SC’s inner wall showing CDH5 and lectin (Lec) labeling, Bottom panels, colocalization of CDH5 and lectin at the AJ. Supplementary Fig. 1 shows all proteins imaged separately. Cyan dashed lines show the direction of flow of tracer into the plane of view (borrowed from organic chemistry depiction of bond direction into paper or screen). C. Quantification of lectin-CDH5 colocalization within the inner wall of SC in eyes perfused at 16 mmHg (mean ± SD, 5.3 ± 2.1 %), 25 mmHg (mean ± SD, 19.1 ± 7%) and 45 mmHg (mean ± SD, 26.9 ± 9.3%). Each of the 96 points represents data for the entire width of a 246 µm lengths of SC from each quadrant (4 tiles per quadrant x 4 quadrants x 6 eyes) at each pressure. One-way ANOVA results, F (2285) = 244.3, p = 1.6 × 10−62. Horizontal line in violin plot is the median and vertical bar the 95%CI. Scale, 25 µm.
