Fig. 3: Site-specific tyrosine phosphorylation (pY658) of CDH5 increases with increasing IOP.
From: FYN regulates aqueous humor outflow and IOP through the phosphorylation of VE-CADHERIN
A Experimental scheme, B Prox1-GFP eye pressure clamped at 16 mmHg, 25 mmHg, or 45 mmHg. a–c the inner wall of SC with GFP and CDH5 labeling, and a’-c’ colocalization of pY658-CDH5 and CDH5 at cell-cell junctions at the indicated pressure. C. Quantification of pYCDH5-CDH5 colocalization within the inner wall of SC in eyes perfused at 16 mmHg (mean ± SD, 7.8 ± 2.2 %), 25 mmHg (mean ± SD, 14 ± 2.4%) and 45 mmHg (mean ± SD, 28 ± 4.6%). Each of the 96 points represents data from a 246 µm length of SC at each pressure (4 tiles per quadrant x 4 quadrants x 6 eyes. One-way ANOVA results, F (2,285) = 946.71, p = 1.3 × 10−126. Horizontal line in violin plot is the median and vertical bar the 95%CI. Fig S2 shows all proteins visualized as individual channels. Scale, 20 µm.
