Fig. 1: D1- and D2-MSNs encode positive and negative valence unconditioned stimuli. | Nature Communications

Fig. 1: D1- and D2-MSNs encode positive and negative valence unconditioned stimuli.

From: Dynamic representation of appetitive and aversive stimuli in nucleus accumbens shell D1- and D2-medium spiny neurons

Fig. 1

A Schematic representation of calcium imaging recordings of GCaMP6f in NAc D1- or D2-MSNs using 1P-miniaturized microscopes; AP: anteroposterior. B Representative expression of GCaMP6f in the NAc, depicting GRIN lens location; scale bar: 250 μm. C Schematic representation of unexpected unconditioned stimulus (US) exposure sessions. US1 session consisted of 39 exposures to 15 ul of 20% of sucrose with 30 s ITI; US2 session consisted of 7 exposures to a 0.5 mA 1 s foot shock with variable ITI of 35–50 s. D Field of view of an example D1-cre mouse showing neurons responsive to US1 (green, left), to US2 (orange, middle) and to both (yellow, right); single traces of tracked neurons in both sessions in a sucrose session (left) and a shock session (right); vertical lines in traces represent events. E Heatmaps of responses of D1-MSNs to US1 and to US2 (nUS1 = 264; nUS2 = 227), and of F D2-MSNs (nUS1 = 164; nUS2 = 100). G Percentage of excited (red), inhibited (blue) and non-responsive (gray) D1-MSNs or H D2-MSNs in response to US1 and US2. I Percentage of each type of response of neurons tracked during US1 and US2 for D1-MSNs and J D2-MSNs. K Heatmap of individual D1-MSNs or L D2-MSNs showing variable responses throughout trials. M Correlation of activity of individual D1-MSNs or N D2-MSNs in response to US1 or US2. O Cartoon depicting population-based linear support vector machine (SVM) decoder (feature: population activity during stimulus; observations: average activity during a given trial; output: US1 or US2). P Classification accuracy for every mouse using population activity of D1- (two-tailed unpaired t-test) or Q D2-MSNs (two-tailed unpaired t-test) during US1 and US2 (0-3 s from US onset) (nD1-cre = 6, nA2A-cre = 6). The decoders were able to correctly identify sucrose and shock trials. Trajectories of trial-by-trial of R D1- or S D2-population responses to US1 and US2; Data from a representative mouse; Dots indicate stimulus onset; US1 and US2 trajectories move in orthogonal directions, supporting differential representation by D1- and D2-MSNs. Data are presented as mean values +/−SEM. **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file. Created in BioRender. Pinto, L. (2024) BioRender.com/p41z755.

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