Fig. 1: In vivo imaging of SARS-CoV-2-infected K18-hACE2 C57BL/6J mice.
From: Neutrophil adhesion to vessel walls impairs pulmonary circulation in COVID-19 pathology
a Survival and body weight changes of SARS-CoV-2-Venus-infected mice. K18-hACE2 C57BL/6J mice were infected with 101–105 PFU of SARS-CoV-2-Venus, and survival and body weight were monitored daily for 10 days. n = 6 biologically independent animals. The results are expressed as the mean ± SD. b Pathophysiological changes in SARS-CoV-2-infected lungs. K18-hACE2 C57BL/6J mice were intranasally infected with SARS-CoV-2-Venus and observed at the indicated timepoints. White arrows indicate infected cells. c In vivo imaging of SARS-CoV-2-infected cells on 4-day post-infection (dpi). (i) and (ii) are enlarged images of the leftmost image. d The number of intravascular neutrophils in SARS-CoV-2-infected lungs. On 6 dpi, SARS-CoV-2-Venus-infected K18-hACE2 C57BL/6J mice were observed, and neutrophils in the pulmonary capillaries in the microscopic field of view were counted. n = 6 biologically independent animals. Each dot represents data from an individual animal and the horizontal bars represent means ± SD. b–d Green indicates virus-infected cells. At the indicated timepoints, fluorescent dextran (white), fluorochrome-conjugated anti-mouse Ly-6G antibody (red), and anti-mouse CD41 (cyan) were i.v. administered to visualize lung architecture, neutrophils, and platelets, respectively. Source data are provided as a Source Data file.
