Fig. 4: GATD3A overexpression in FLSs alleviates OA development in mice.
a Experimental design diagram of intra-articular injection of rAAV-Vec or rAAV-GATD3A in mice (Created in BioRender. Shen, K. (2024) https://BioRender.com/w90b978). b Representative images of immunohistochemistry staining of GATD3A in the knee joint from mice injected with rAAV-Vec or rAAV-GATD3A. n = 7 independent biological samples. Scale bar: 50 µm. c Representative images of immunofluorescent staining of vimentin, GATD3A, and p16INK4a in the knee joint from sham or DMM-induced OA mice after intra-articular injection with rAAV-Vec or rAAV-GATD3A. Enlarged images were shown below. n = 7 independent biological samples. Scale bar: 50 µm. d 3D reconstructed images of micro-CT of knees and osteophytes (arrows pointed), and statistical analyses of the number of osteophytes. n = 7 independent biological animals. e Representative coronal plane images of knee joints obtained from micro-CT data analyses. f Quantification of bone volume per total volume (BV/TV), and trabecular thickness (Tb. Th) obtained from micro-CT data. n = 7 independent biological animals. g Representative images of H&E staining, Safranin O/Fast green staining and Toluidine Blue staining for knee joints from sham or DMM-induced OA mice after intra-articular injection with rAAV-Vec or rAAV-GATD3A. n = 7 independent biological animals. Scale bar: 100 µm. The severities of synovitis and OA phenotype were assessed through synovial inflammation score (h) and OARSI score system (i). n = 7 independent biological animals. All data were presented as the means ± SD. P values were determined by one-way ANOVA with Tukey’s multiple-comparisons. P < 0.05 was considered statistically significant. Source data are provided as a Source Data file.
