Fig. 6: GATD3A interacts with MDH2, a key enzyme of the TCA cycle. | Nature Communications

Fig. 6: GATD3A interacts with MDH2, a key enzyme of the TCA cycle.

From: GATD3A-deficiency-induced mitochondrial dysfunction facilitates senescence of fibroblast-like synoviocytes and osteoarthritis progression

Fig. 6

a The heatmap of significantly different enzymes in the TCA cycle based on proteomics assay for Nor-FLSs infected with ShNC or ShGATD3A lentivirus. b IP/MS analysis indicated that MDH2 is an interacting protein that binds to GATD3A. c Co-IP assay to verify the interaction between endogenous GATD3A and MDH2 in FLSs. n  =  3 independent biological replicates. d Representative images of immunofluorescence to verify the co-localisation of endogenous GATD3A and MDH2 in FLSs. n  =  5 independent biological replicates. Scale bar: 10 µm. e Co-IP assay to verify the interaction between exogenous Flag-GATD3A and His-MDH2 in HEK 293 T cells. n  =  3 independent biological replicates. f Representative images of immunofluorescence to verify the co-localisation of exogenous Flag-GATD3A and His-MDH2 in HEK 293 T cells. n  =  5 independent biological replicates. Scale bar: 10 µm. g Design of GATD3A and MDH2 truncations. h Co-IP assay showing the interactions between Flag-GATD3A and His-MDH2 in HEK 293 T cells. n  =  3 independent biological replicates. i Molecular docking predicted the docking sites of GATD3A and MDH2. j Docking sites of GATD3A and MDH2, and the mutations of binding sites between GATD3A and MDH2. k Co-IP assay showing the interactions between Flag-tagged mutated GATD3A and His-tagged mutated MDH2 in HEK 293 T cells. n  =  3 independent biological replicates. Source data are provided as a Source Data file.

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