Fig. 4: Genotype-phenotype correspondence.

a Schematic showing how to prepare droplets containing homogeneous genotypes and inquire whether homogeneous phenotypes are expressed in each droplet. b Expression of GFP and RFP at 200 min from the initial image acquisition. The first column at the left end shows the sample prepared in the manner depicted in (a). The second column shows the condition where premixed gfp DNA solution and rfp DNA solution were added. The third and fourth columns show conditions where gfp DNA and rfp DNA are solely added, respectively. The bottom row shows marginal plots, where scatter plots showing GFP and RFP fluorescence from each droplet are combined with histograms for each axis. n values indicate the number of droplets plotted for each condition. The experiment was repeated more than five times with similar results. The scale bar represents 10 µm. See also Supplementary Fig. 7b for line profiles.