Fig. 4: TANB77 abundance in the murine gut microbiome correlates with anti-PD-1 (aPD-1) therapy response.

a TANB77 abundance in JAX and TAC phenotype mice. Significance was evaluated by a two-tailed Mann Whitney U test. The center line of the boxplot represents the median, the bounds of the box represent the 25th, and 75th percentiles, and the whiskers represent the minimum and maximum values within the 1.5 interquartile range from the lower and upper quartiles. b Changes of TANB77 abundance in guts of “JAX to TAC” (blue, n = 6) and “TAC to TAC” (red, n = 6) mice after transplantation. The plot denotes average TANB77 abundance ± SEM. Statistical significance was calculated by a two-tailed Mann-Whitney U test. c Relative abundance of TANB77 in JAX donor (navy, n = 4) and TAC recipient mice on day 14 (D + 14). The plot denotes average TANB77 abundance ± SEM. Statistical significance was calculated by a two-tailed Mann-Whitney U test. d Schematic timeline of the mouse experiment relative to the tumor injection date. e Tumor growth curve of high- and low-TANB77 mice subjected to aPD-1 or isotype treatment. Data points and error bars represent the mean ± SEM of tumor volume from two independent experiments (aPD1 high-TANB77 = 9, aPD1 low-TANB77 = 12, Isotype high-TANB77 = 12, Isotype low-TANB77 = 9). Significance was evaluated by two-way ANOVA using Tukey’s multiple comparison post hoc test (all significant comparisons are shown). f Correlation between normalized tumor growth and relative abundance of the UMGS1663 genus in aPD-1 treated mice from day 11 (D + 11) to D + 14 (left), from D + 14 to D + 17 (center), and from D + 17 to D + 20 (right). The plots share y-axis. Solid lines denote linear regression. Source data are provided as a Source Data file. (n.s.: not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).