Fig. 4: Menopause induces significant changes in gene regulation and -expression in epithelial cells.
From: A cell atlas of the human fallopian tube throughout the menstrual cycle and menopause
A Pre- and post-menopausal SE cells identified using UMAP profiling. The pre-menopausal fallopian tube (n = 10) is characterized by three SE subtypes (SE1 pre, SE2 pre, and SE3 pre), while the post-menopausal fallopian tube (n = 7) is characterized by two SE subtypes (SE post and SE post-specific). The number of cells identified in each subtype is shown in the panel (scRNA-seq). B Dot plot showing normalized gene expression levels of selected markers associated with proliferation, stemness, and PEG-cells in pre- and postmenopausal SE subtypes based on scRNA-seq data. C Dot plot showing normalized gene expression levels of hormone receptors in pre- and postmenopausal SE subtypes based on scRNA-seq data. D Heatmap showing the average expression of target genes in SE pre- and post-menopausal subtypes at the individual sample level based on scRNA-seq data (ampulla and fimbria). E Heatmap showing the absolute transcription factor activities in SE subtypes using the scATAC-seq data. F Heatmap showing relative transcription factor activities in SE subtypes using scATAC-seq data. The Z-score is obtained by centering and re-scaling across SE subtypes for each TF. G Dot-plot showing the enrichment of selected gene ontology (GO) terms for each SE subtype based on scRNA-seq. FDR-corrected p-values are shown. H Spatial transcriptomics profiling of normal pre-menopausal fallopian tube fimbria (n = 1) using 10x Genomics Visium. Pre-menopausal fallopian tube fimbria were analyzed for spatial expression of KRT7, KRT15, TUBB, OVGP1 and INSR1.
