Fig. 5: Salidroside supplementation enhances ovarian angiogenesis and blood supply in aged ovaries. | Nature Communications

Fig. 5: Salidroside supplementation enhances ovarian angiogenesis and blood supply in aged ovaries.

From: Physiological premature aging of ovarian blood vessels leads to decline in fertility in middle-aged mice

Fig. 5

a Live-cell imaging analysis tracking the growth of blood vessels of Tek-CreERT2;mTmG follicles from 12-month-old females with Sal, Ft1, Dosk or vehicle. b Relative growth rate of vascular area in follicles during 24 h of in vitro culture, revealing Sal as the most significant component for increasing blood vessel growth in cultured follicles (Control: n = 10 follicles from 3 mice, Sal: n = 8 follicles from 3 mice, Ft1: n = 9 follicles from 3 mice, Dosk: n = 8 follicles from 3 mice). c Sal treatment strategy in 12-month-old females. After 7 days of Sal (300 mg/kg BW, once a day) or vehicle treatment, females were evaluated for their reproductive ability or the angiogenesis intensity. d Tip cells on growing follicles after 7 days of Sal or vehicle treatment (left), quantifying the number of tip cells (right) showing a significant increase in tip cells on growing follicles after 7 days of Sal treatment (n = 5 mice). e Whole-mount reconstruction of ovarian blood vessels (left) after 7 days of Sal or vehicle treatment at 12 months and quantification analysis (right) showing a slight elevation in ovarian vessel density after Sal treatment compared to the control (n = 5 mice). f Intensity of Evans blue (arrowheads) in ovaries (left) of 12-month-old females after Sal or vehicle treatment. Quantification of the EB signal (right) indicating Sal treatment significantly increased the blood supply to the ovaries (n = 4 mice). g EB signals in follicles from the females with or without Sal treatment (left), and quantitation of EB abundance in follicles showing Sal treatment significantly enhanced the blood supply to the follicles (right, Control: n = 8 follicles from 3 mice, Sal: n = 7 follicles from 3 mice). Data are presented as the means ± SD. Data were analyzed by 2-tailed unpaired Student’s t-test without adjustments; *** P < 0.001, ** P < 0.01, * P < 0.05. Scale bars: 100 μm (a and g), 10 μm (d), 500 μm (f). Source data are provided as a Source Data file including exact P values.

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