Fig. 2: 3D genome structures calculated from K-matrices recapitulate chromosomal fusions in the engineered cells with different fusion events.

a Representative models show tethering of the fused chromosomes in R1n13 cell lines, whereas separated in O48 cells. Fused chromosomes were colored as indicated accordingly; other chromosomes were shown as gray lines; Centromeres were displayed as balls. b, c Euclidean distance between fused chromosomes (b) or centromeres (c) in R1n13 and O48 cell lines. Each data point represents the average distance in 100 models of each sample repeats (3 repeats). p-value was indicated, two-sided t-test. d Chromosome distance distribution between fused chromosomes in each model of wild-type O48 and R1n13 cell line repeats. e Representative models show tethering of the fused chromosomes in telomere-centromere fused T12 (chr1 + 2) cell line, cleavage of chr1 was clearly observed, and the proximal arm was fused with chr17. Here, telomere refers to the telomere located at the end of the chromosome opposite to the centromere end. Centromeres were displayed as balls. f, Distance between telomere of chr1 and centromere of chr2 in T12 cell line (2 repeats, 100 models each). g, h Cross-section view of expression profile (g) and A/B compartment distribution (h) in R1n13 and O48 cell lines. Blue, low expression area or B compartments; Red, high expression area or A compartments. i Surface view and cross-section view of A/B compartment distribution in Rod neurons.