Fig. 3: The pro-inflammatory landscape of CD14+ monocytes in mediating MPN disease.

a Plasma cytokine expression across 180 samples representative of PV (n = 37), MF (n = 107), sAML (n = 6) patients, and healthy donors (n = 30). Statistics were assessed by a two-tailed Mann-Whitney U test. Data are presented as mean values +/− SD. b Schematic of the WashU Cohort CD14 + RNA-seq encompassing 68 primary samples representative of PV (n = 8), ET (n = 15), MF (n = 31), and NBM (n = 14). c Principal component analysis of primary samples in the WashU Cohort CD14 + RNA-seq by gene expression. d Volcano plot showing DEGs by DESeq2 analysis comparing MF to NBM CD14 + monocytes. Y-axis denoting -Log₁₀ adj. p-value. X-axis denoting Log₂ expression fold change. e Boxplot showing mRNA expression of genes of interest from the WashU Cohort CD14 + RNA-seq. Statistics assessed by two-tailed student’s t test between MF and NBM. Sample numbers are denoted in panel (b). Boxplot showing median, upper, and lower quartiles with whiskers representing highest and lowest values excluding outliers, and potential outliers. f Gene set enrichment analysis of top altered pathways comparing MF to NBM CD14 + monocytes. NES = normalized enrichment score. NOM p-value: Nominal p-value denoting the statistical significance of the enrichment score.