Fig. 4: PV to MF disease transformation at single cell resolution. | Nature Communications

Fig. 4: PV to MF disease transformation at single cell resolution.

From: RSK1 is an exploitable dependency in myeloproliferative neoplasms and secondary acute myeloid leukemia

Fig. 4

a Schematic of clinical information and sample collection of patient 551599 through disease transformation. b Cell population proportion of 551599 PBMC samples by CyTOF. c tSNE plots showing surface CD14 + protein and key cytokine expression of 551599 samples. d Percent positive cytokine expression in 551599 CD14 + monocytes across disease stages and healthy peripheral blood donor (NPB) control by CyTOF. e Fold change of cytokine expression in 551599 CD14 + monocytes following 4h ex vivo treatment with 5 μM ruxolitinib by CyTOF. f Expression of signaling proteins in 551599 CD34 + HSCs and CD14 + monocytes at MF pPV compared to Early PV by CyTOF. Values represent the median arcsinh ratio. g UMAPs of 551599 from scRNA-seq by disease state and cell identity. h Expression dot plot of key genes from 551599 across disease states by scRNA-seq. i Trajectory branching of HSPC and CD14 + monocyte populations. j Pseudotime trajectory analysis of TNF, CCL3, and RPS6KA1 expression across disease progression.

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