Fig. 4: Long-range organization of crypt-like domains.

a Delaunay triangulation. Immunofluorescence image of the epithelial monolayer stained for Ki67, F-Actin and cell nuclei (DNA). Scale bar: 200 μm. b Distribution of the distance between crypts, dashed line corresponds to a Gaussian fit. Mean ± SEM. N = 6 experiments. c Angle distribution, dashed line corresponds to the fitting using a Gaussian distribution function. Mean ± SEM. N = 6 experiments. d Organoid-derived single cells seeded on treated rBM with the Wnt3a micropattern at 48 h of culture. Representative fluorescence images of F-Actin, cell nuclei (DNA) and Wnt3a (left panel), cell area segmentation (middle panel), and cell area contour map (right panel). Scale bars: 200 μm. e Upper panel: Distribution of the distance between crypts, dashed line corresponds to the Gaussian fit of the control distribution (in b). Mean ± SEM. N = 3 experiments. Lower panel: Angle distribution, dashed line corresponds to the Gaussian fit of the control distribution (in c). Mean ± SEM. N = 3 experiments. f Organoid-derived single cells seeded on treated rBM with the EphrinB1 micropattern at 48 h of culture. Representative fluorescence images of F-Actin, cell nuclei (DNA) and EphrinB1 (left panel), cell area segmentation (middle panel), and cell area contour map (right panel). Scale bars: 200 μm. g Upper panel: Distribution of the distance between crypts, dashed line corresponds to the Gaussian fit of the control distribution (in b). Mean ± SEM. N = 4 experiments. Lower panel: Angle distribution, dashed line corresponds to the Gaussian fit of the control distribution (in c). Mean ± SEM. N = 4 experiments. Source data are provided as Source Data file.