Fig. 5: Effect of pattern dimensions on long-range epithelia organization.

a Organoid-derived single cells seeded on treated rBM with the Wnt3a micropattern spaced 150 µm, 200 µm and 400 µm respectively at 48 h of culture. Representative fluorescence images of F-Actin, cell nuclei (DNA) and Wnt3a (upper panels). Representative cell area contour map (lower panel). Scale bars: 200 μm. b Graph plotting the probability of Wnt3a dots of hosting crypt and villus as a function of dots distance. Mean ± SEM. N = 3 experiments. c Distribution of the distance between crypts on Wnt3a patterns, dashed line corresponds to the Gaussian fit of the control distribution. Mean ± SEM. N = 3 experiments. d Distribution of the angles between crypts on Wnt3a patterns, dashed line corresponds to the Gaussian fit of the control distribution. Mean ± SEM. N = 3 experiments. e Organoid-derived single cells seeded on treated rBM with the EphrinB1 micropattern spaced 150 µm, 200 µm and 400 µm respectively at 48 h of culture. Representative fluorescence images of F-Actin, cell nuclei (DNA) and EphrinB1 (upper panels). Representative cell area contour map (lower panel). Scale bars: 200 μm. f Graph plotting the probability of EphrinB1 holes of hosting crypt and villus as a function of holes distance. Mean ± SEM. N = 3 experiments. g Distribution of the distance between crypts on EphrinB1 patterns, dashed line corresponds to the Gaussian fit of the control distribution. Mean ± SEM. N = 3 experiments. h Distribution of the angle between crypts on EphrinB1 patterns, dashed line corresponds to the Gaussian fit of the control distribution. Mean ± SEM. N = 3 experiments. Source data are provided as Source Data file.