Fig. 2: Encapsulation of siRNA into IPMs.
From: Ionizable polymeric micelles (IPMs) for efficient siRNA delivery
a Schematic representation of different ionizable oligomers (IO) structures. b Schematic illustration of IPM structure. c Particle size distribution of IPMs formed by different IOs with PEG5000-PLA8000. d Particle size, PDI, and encapsulation efficiency of IPMs at different N/P ratios (n = 3 samples). IPM achieves the smallest particle size and PDI, along with higher encapsulation efficiency, at N/P = 5.45, with a zeta potential close to 0. e Particle size, PDI, encapsulation efficiency, and zeta potential of IPMs formed by different IOs (PEG5000-PLA8000) (n = 3 samples). IPM (IOA2) exhibits the smallest particle size, PDI, and highest encapsulation efficiency. PEG5000-PLA8000 abbreviated as P5P8, PEG2000-PLA5000 abbreviated as P2P5, PEG2000-PLA2000 abbreviated as P2P2. f Particle size, PDI, encapsulation efficiency, and zeta potential of IPMs formed by different segment molecular weights of PEG-PLA (IOA2) (n = 3 samples). IPM (PEG5000-PLA8000) shows the smallest particle size and highest encapsulation efficiency. g TEM images of IPMs formed by different IOs with PEG5K-PLA8K and nucleic acid stained by thionine. The morphology appears spherical, with IPM (IOA2) having the smallest particle size, around 100 nm, and a higher concentration of RNA with a more punctate distribution. Values represent mean ± standard deviation (SD).
