Fig. 2: Construction of the initial ω-AmDDA cell factory.
From: De novo biosynthesis of nylon 12 monomer ω-aminododecanoic acid
The left panels show the pathway modules constructed in strains B1-1 (R-BTE) and B1-1 (R-BTE/E-M1-3/C-M2-2). The upper right panel shows the GC spectra of the culture broth of strain B1-1 (R-BTE) and the DDA standard. The lower right panel shows the HPLC spectra of the culture broth of strains B1-1 (R-BTE/E-M1-3/C-M2-2) and B1-1 (pRSF/E-M1-3/C-M2-2) together with the ω-AmDDA standard. The OD600 at the time of induction was approximately 1.0 and the fermentation time was 24 h after induction. DDA dodecanoic acid, FA13 tridecanoic acid, ω-OHDDA ω-Hydroxydodecanoic acid, ω-AmDDA ω-aminododecanoic acid.
