Fig. 4: Modular pathway engineering for improving ω-AmDDA de novo synthesis.
From: De novo biosynthesis of nylon 12 monomer ω-aminododecanoic acid
A Modular engineering of DDA supply (in gray) and conversion (in blue). B Effect of DDA supply engineering on ω-AmDDA synthesis. C Effect of regulating DDA formation and hydroxylation on ω-AmDDA synthesis. The numbers in the tables indicate the copy numbers of the corresponding modules integrated into the genome while indicating the presence of the corresponding modules on the plasmid. The OD600 at the time of induction was approximately 1.0 and the fermentation time was 24 h after induction. DDA dodecanoic acid, ω-OHDDA ω-hydroxydodecanoic acid, ω-ODDA ω-oxododecanoic acid, ω-AmDDA ω-aminododecanoic acid, FAS M acyl-ACP synthesis module, ACA M acetyl-CoA activation module, GLY M glycolysis module, R-BTE fatty acid synthesis pathway module, EApr-M1 DDA hydroxylation module with AprR marker, C-M2-A oxidation and transamination module. All values presented are the means of three biological replicates, and error bars represent standard deviations. Source data are provided as a Source Data file.
