Fig. 6: Engineering of CYP153A-NCP.
From: De novo biosynthesis of nylon 12 monomer ω-aminododecanoic acid
A Tunnel recognition and analysis of the heme domain of CYP153A-NCP; B Hydroxylation of DDA by CYP153A-NCP mutants designed by alignment. C Schematic diagram of the colorimetric method for in situ detection of DDA terminal hydroxylation; D Mutants generated by combinatorial mutagenesis. DDA dodecanoic acid, ω-OHDDA ω-hydroxydodecanoic acid, ω-ODDA ω-oxododecanoic acid, ω-AmDDA ω-aminododecanoic acid. All values presented are the means of three biological replicates, and error bars represent standard deviations. Source data are provided as a Source Data file.
