Fig. 8: Enhancing dimerization of CYP153A-NCP. | Nature Communications

Fig. 8: Enhancing dimerization of CYP153A-NCP.

From: De novo biosynthesis of nylon 12 monomer ω-aminododecanoic acid

Fig. 8

A Different intermolecular affinity-enhancing structures. B Hydroxylation of DDA by fusion constructs with different oligomerization enhancing domains. C Effects of enhancing DDA hydroxylation on the de novo synthesis of ω-AmDDA. Control, PDANR (EApr-M1-B3/C-M2-A). The OD600 at the time of induction was approximately 1.0 and the fermentation time was 18 h after induction. DDA dodecanoic acid, ω-OHDDA ω-hydroxydodecanoic acid. All values presented are the means of three biological replicates, and error bars represent standard deviations. Source data are provided as a Source Data file.

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