Table 1 Per-residue energy decomposition (ΔGbind) of CYP153A mutants G307A and G307A/A231R/Q129A/S140Qa
From: De novo biosynthesis of nylon 12 monomer ω-aminododecanoic acid
Residue | G307A (kcal/mol) | G307A/A231R/Q129A/S140Q (kcal/mol) |
|---|---|---|
128 | 0.05 ± 0.09 | 0.11 ± 0.03 |
129 | −0.17 ± 1.68 | −0.19 ± 0.05 |
130 | −0.92 ± 0.34 | −0.64 ± 0.11 |
131 | −0.92 ± 0.25 | −0.27 ± 0.07 |
132 | −0.04 ± 0.02 | −0.03 ± 0.02 |
133 | 0.04 ± 0.03 | 0.01 ± 0.02 |
134 | 0.19 ± 0.17 | 0.22 ± 0.24 |
135 | −1.08 ± 0.35 | −1.04 ± 0.29 |
136 | 0.11 ± 0.45 | 0.17 ± 0.11 |
137 | 0.42 ± 0.18 | 0.26 ± 0.21 |
138 | −0.15 ± 0.07 | −0.05 ± 0.03 |
139 | −1.42 ± 0.65 | 0.11 ± 0.13 |
140 | −1.19 ± 0.48 | −2.57 ± 0.48 |
141 | −1.31 ± 1.3 | −1.43 ± 0.65 |
142 | 0.87 ± 0.88 | 0.49 ± 0.29 |
143 | −0.64 ± 0.28 | −0.66 ± 0.44 |
144 | −0.04 ± 0.01 | −0.06 ± 0.03 |
145 | −0.6 ± 0.21 | −0.94 ± 0.21 |
146 | −0.11 ± 0.04 | −0.1 ± 0.03 |
225 | 0.07 ± 0.1 | 0.11 ± 0.09 |
226 | 0.45 ± 0.2 | 0.41 ± 0.29 |
227 | −0.19 ± 0.13 | −0.43 ± 0.2 |
228 | 1.07 ± 0.8 | −0.13 ± 0.36 |
229 | −0.33 ± 0.29 | −0.14 ± 0.14 |
230 | −0.12 ± 0.05 | −0.07 ± 0.02 |
231 | −0.29 ± 0.16 | −8.22 ± 2.84 |
232 | 0 ± 0.01 | 0 ± 0.02 |
233 | 0 ± 0.06 | −0.04 ± 0.02 |
234 | −0.15 ± 0.12 | −0.12 ± 0.04 |
235 | −0.25 ± 0.1 | −0.08 ± 0.06 |
302 | −0.09 ± 0.1 | −0.45 ± 0.31 |
303 | −0.34 ± 0.19 | −0.73 ± 0.3 |
304 | 0.1 ± 0.06 | 0.08 ± 0.09 |
305 | 0.05 ± 0.07 | −0.09 ± 0.07 |
306 | −0.61 ± 0.41 | −1.43 ± 0.39 |
307 | −0.3 ± 0.39 | −0.61 ± 0.45 |
308 | −0.03 ± 0.04 | −0.06 ± 0.05 |
309 | −0.33 ± 0.13 | −0.25 ± 0.08 |
310 | 0.99 ± 0.43 | 0.77 ± 0.36 |
311 | −0.42 ± 0.17 | −0.56 ± 0.16 |
