Fig. 2: Impaired cortical plate formation with loss of Tbr2+ intermediate progenitors in Ohiam/m mutant brain.
A–L Sections of the brain of E16.5 control A–D and Ohiam/m mutant mice with mild E–H or severe I–L forebrain hypoplasia were stained with cresyl violet A, E, I to delineate the brain tissue architecture. Antibody staining was conducted for markers of apical progenitor cells (Pax6 in B, F, J), intermediate progenitor cells (Tbr2 in C, G, K), and merged image showing both markers D, H, L. Boxed regions in panels A,E,I are shown in magnified view under darkfield illumination in B–D, F–H, J–L respectively. M–T Section of the cortical plate of E16.5 wildtype (M–P) and mutant (Q–T) mouse brain was stained with antibodies to Satb2, marker for cortical layers II–IV (M, Q), Ctip2 for cortical layers V–VI (O, S), and Tbr1 (N, R) for cortical layer VI, and merged image showing all three markers (P, T). U–Y Quantification of number of cells stained by Pax6 (U, P = 0.9639), Tbr2 (V, P < 0.0001), Ctip2 (W, P = 0.2598), Tbr1 (X, P = 0.3494), and Satb2 (Y, P < 0.0001) in the brain of 5 Ohiam/m mutant and 5 littermate controls. Graphs represent mean ± SD. P-values were calculated by 2-tailed, unpaired t-test. Scale bars in A–L indicate 500 µm. Scale bars in M–T indicate 100 µm. CP cortical plate, SVZ subventricular zone, VZ ventricular zone. Source data are provided as a Source Data file.
