Fig. 3: Dietary methionine restriction enhances drug efficacy by reducing YAP R124me2a.

a The cell viability of YAP-, YAP-S127A-, or YAP-R124F-overexpressed Huh-7 cells cultured in methionine-deprived medium (MD) and treated with sorafenib for 24 h. b The cell viability of YAP-, YAP-S127A-, or YAP-R124F-overexpressed HCT 116 cells cultured in MD and treated with oxaliplatin for 48 h. c Schematic representation of the experimental design in Balb/c nude mice to measure the combination effect of dietary methionine restriction and sorafenib in stable YAP-R124F- or YAP-S127A-overexpressed cells. d Representative image of tumor nodules in different conditions described in Fig. 3c. e, f The statistical analysis of tumor volume (e) and tumor weight (f) in different groups described in Fig. 3c. g, h Representative images (g) and quantitation (h) of ki-67 staining in tumors from different groups were shown. scale bar = 50 μm. i, j The relative expression of CTGF (i) and CYR61 (j) was analyzed using real-time PCR. Statistical analyses were performed using one-way ANOVA with multiple comparisons (a, b, f, h–j) or two-way ANOVA with multiple comparisons (e). Data were presented as mean ± SEM for e and mean ± SD for a, b, f, h, i, j. n = 3 (a, b) or 6 (e, f, h, i, j) biologically independent samples. Source data are provided as a Source Data file.