Fig. 6: Disrupting the positive feedback between YAP R124 methylation and SLC43A2 improves drug sensitivity.

a The level of YAP R124 methylation in Huh-7 cells and HCT 116 cells treated with BCH (1 mM) for 72 h. The data represents the relative ratio of YAP R124me2a to YAP. b, c The transcriptional levels of YAP and YAP target genes in Huh-7 cells (b) and HCT 116 cells (c) were treated with BCH (1 mM) for 72 h. d The cell viability of YAP-, YAP-S127A-, or YAP-R124F-overexpressed Huh-7 cells treated with sorafenib and BCH (1 mM) for 24 h. e The cell viability of YAP-, YAP-S127A-, or YAP-R124F-overexpressed HCT 116 cells treated with oxaliplatin and BCH (1 mM) for 48 h. f Schematic representation of the experimental design in Balb/c nude mice to measure the combination effect of BCH and sorafenib in stable YAP-R124F- or YAP-S127A-overexpressed cells. n = 5 per group. g Representative image of tumor nodules in different conditions described in Fig. 6f. h The statistical analysis of tumor weight in different conditions is described in Fig. 6f. i, j The expression of CTGF (i) and CYR61 (j) was analyzed in different conditions described in Fig. 6f. k The quantitation of ki-67 staining in tumors from different groups was analyzed. Statistical analyses were performed using two-sided unpaired Student’s t-test (b, c) or one-way ANOVA with multiple comparisons (d, e, h–k). Data were presented as mean ± SD. n = 3 (b–e) or 5 replicates (h–k) biologically independent samples. The data presented in (a) are representative of three independent experiments. Source data are provided as a Source Data file.