Fig. 1: SNA is synchronized translaminarly at P7.
a Schematic depicting the placement of microprism in the barrel cortex. b Image of S1 at P14 after microprism was removed. Barrels are indicated by VGLUT2 staining. Arrow indicates microprism placement. Scale bar = 1 mm. c Example ROIs from the same (magenta, violet and orange) and different columns (blue and green) marked on a 2-photon FOV at P7. ROIs are 25 × 25 μm squares from dividing the FOV by a grid. Scale bar = 50 μm. d dF/F transients measured in the corresponding ROIs shown in (c). e Example SNA at 4 distinct time points during one video. Scale bar = 50 μm. f ROIs are color-coded using a k-means clustering algorithm. Best clustering (highest silhouette values) corresponds to clusters organized in columns as shown. g Silhouette values of the ROIs organized in columns versus randomized ROIs (0.12 ± 0.011 vs. -0.0047 ± 0.0035, two-sided paired t-test, p = 0.0008). h Pairwise correlation between ROIs, sorted by columns. The three corresponding clusters in (f) are labeled. i The percentage of pairs that are significantly correlated within the same column, compared to the pairs in the entire FOV (80.03 ± 6.22 vs. 38.41 ± 4.75, two-sided paired t-test, p = 0.0004). n = 5 videos from 5 mice. ***p < 0.001. Box plot (g, i) whiskers indicate the minimum and maximum values. Bounds of the box are the 1st and 3rd quartile and the center line indicates the median. Source data are provided as a Source Data file.
