Fig. 4: Synaptic Strength of L4 versus L5 inputs to L2/3 pyramidal neurons from 1st to 3rd PNW. | Nature Communications

Fig. 4: Synaptic Strength of L4 versus L5 inputs to L2/3 pyramidal neurons from 1st to 3rd PNW.

From: Translaminar synchronous neuronal activity is required for columnar synaptic strengthening in the mouse neocortex

Fig. 4

a Schematic depicting experimental setup for comparing the strength of L5 inputs to L2/3 pyramidal neurons in the same versus neighboring column in 1st PNW. b Example DIC image of L4 barrels. Patch pipette is positioned in L2/3. M: medial, L: lateral. Scale bar = 100 μm. c Example EPSC traces elicited by electrically stimulating L5 neurons and recorded in L2/3 pyramidal neurons in the same (right) versus neighboring (left) barrel column. d EPSC response amplitudes in L2/3 pyramidal neurons in the two locations (same vs. neighbor, 128.61 ± 12.67 vs. 55.07 ± 9.98, two-sided paired t-test, p = 0.0074). n = 7 cells for each location. e Schematic depicting experimental setup for comparing the strength of L4 versus L5 inputs to L2/3 neurons in the same barrel column. f Example EPSCs recorded in the same L2/3 neuron while stimulating L4 (top) versus L5 (bottom) at the end of 1st, 2nd, and 3rd PNW. g Quantification of peak amplitudes of the first responses of the paired pulse. 1st and 2nd PNWs: n = 16 pairs, 3rd PNWs: n = 15 pairs. (1st and 3rd PNWs: two-sided Wilcoxon test, 105.87 ± 20.72 vs. 29.79 ± 7.28, p = 0.0063, and 104.47 ± 14.82 vs. 254.13 ± 48.25, p = 0.0099 respectively; 2nd PNW: two-sided paired t-test, 94.83 ± 10.70 vs. 227.04 ± 44.70, p = 0.0099). h Contribution of L5 in total L4 and L5 inputs. 1st and 2nd PNWs: n = 16 cells, 3rd PNWs: n = 15 cells. (One-way ANOVA with Bonferroni’s test: 1st vs. 2nd, 71.26 ± 6.60 vs. 37.29 ± 5.34, p = 0.0004; 1st vs. 3rd, 35.39 ± 5.44, p = 0.0003.) Bars represent means ± SEM. i Paired-pulse ratio (PPR) across the three PNWs for L5 (left) versus L4 (right) stimulation (Kruskal-Wallis test: L5 1st to 3rd PNWs: 0.99 ± 0.095 vs. 0.98 ± 0.055 vs. 0.96 ± 0.28, p = 0.33; L4 1st to 3rd PNWs, 0.85 ± 0.12 vs. 0.9932 ± 0.038 vs. 1.01 ± 0.045, p = 0.12). 1st PNW, n = 20 cells; 2nd PNW, n = 19 cells; 3rd PNW, n = 17 cells. **p < 0.01, ***p < 0.001. Box plot (d, g, i) whiskers indicate the minimum and maximum values. Bounds of the box are the 1st and 3rd quartile and the center line indicates the median. Source data are provided as a Source Data file.

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