Fig. 1: Effects of rdm16 mutations on thermotolerance and the splicing of HSF transcripts. | Nature Communications

Fig. 1: Effects of rdm16 mutations on thermotolerance and the splicing of HSF transcripts.

From: Cooperative condensation of RNA-DIRECTED DNA METHYLATION 16 splicing isoforms enhances heat tolerance in Arabidopsis

Fig. 1

a Diagram of the heat treatment used in this study. CK, control treatment at 22 °C; AHT, acquired heat treatment. b, e Representative photographs of rdm16 mutant seedlings and wild type under CK or AHT conditions. c, g Relative electrolyte leakage of wild type and rdm16 mutant seedlings after a 6-day recovery from heat treatment. Interactive P-value between genotype and treatment is <0.001, suggesting that the rdm16 mutants are hypersensitive to heat treatment. d, f Survival rates of wild type and rdm16 mutant seedlings after a 6-day recovery from heat treatment. h Relative RDM16 transcript levels in Col-0, rdm16-4, and rdm16-4 RDM16pro:RDM16-GFP (RDM16:RDM16-GFP) transgenic lines, as determined by RT-qPCR. i Representative photographs of Col-0, rdm16-4, and RDM16:RDM16-GFP seedlings under CK or AHT conditions. j Survival rates of Col-0, rdm16-4, and RDM16:RDM16-GFP seedlings after heat treatment. k Relative electrolyte leakage of Col-0, rdm16-4, and RDM16:RDM16-GFP seedlings. l Splicing efficiency of transcripts for the indicated heat stress–responsive genes in 8-day-old Col-0, rdm16-4, and RDM16:RDM16-GFP seedlings subjected to heat stress as indicated (TR1, 37 °C for 30 min; TR2, 30 min 37 °C, 30 min 22 °C, and 80 min 45 °C). The PCR products either spanned exon–exon junctions to detect spliced transcripts or exon–intron junctions to detect unspliced transcripts. Splicing = spliced/(spliced + unspliced). Data in (c, d, f, g, h, j, k and l) are means ± standard deviation (SD), n = 3. Different letters in (c, g, k and l) indicate a significant difference by two-way ANOVA with post hoc Holm–Šídák test (P < 0.05). In (d, f), significant differences were determined using two-sided Student’s t-test (* P < 0.05; *** P < 0.001). Different letters in (h, j) indicate a significant difference by one-way ANOVA with post hoc Tukey HSD test (P < 0.05). Experiments in (b–k) were performed at least three times with similar results. Experiments in (l) were performed at least twice with similar results.

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