Fig. 5: DCAF13-mediated K63-linked polyubiquitination of RPA194 promotes rDNA transcription.

a, b Western blot analysis of the ubiquitination levels of RPA194 in MDA-MB-231 cells subjected to DCAF13 knockdown (a) or overexpression (b), followed by HA-Ub transfection for 48 hours. c Western blot analysis of the ubiquitination levels of RPA194 in HeLa cells co-transfected with full-length DCAF13 or domain-depleted DCAF13, along with HA-Ub. d Western blot analysis of the ubiquitination levels of RPA194 in MDA-MB-231 cells transfected with either scramble or siDCAF13 for 24 hours, followed by a subsequent 24-hour transfection with HA-ubiquitin (Lys48 or Lys63-specific). e Western blot analysis of K63-linked ubiquitination levels of RPA194 in HeLa cells subjected to serum starvation and subsequent serum activation. f Western blot analysis of ubiquitination levels of RPA194 in HeLa cells transfected with either scramble or siUSP36 for 24 hours, followed by an additional 24-hour transfection with HA-K48 or HA-K63. g Co-IP assays on HeLa cells transfected with pKO-SFB-USP36 to explore interactions between RPA194 and USP36. h IF assays detecting the localization of USP36 and RPA194 in HeLa cells treated with DMSO, ActD, or transfected with siUSP36. Scale bars, 5μm (h). Source data are provided as a Source Data file.