Fig. 6: Transcriptome profiling coupled with EBE prediction reveals a pectin lyase as an additional target of Tal7b, contributing to Xcm water-soaked lesion development.
From: Activation of three targets by a TAL effector confers susceptibility to bacterial blight of cotton
A Venn diagram of Tal7b-induced genes by RNA-sequencing (RNA-seq) analysis and Tal7b-candidate target genes by TALE-NT 2.0-based EBE predication with a score ratio cutoff 4.0. B Tal7b RVDs and predicted EBEs. Colored boxes depict the RVDs of each repeat of Tal7b, with the number above indicating the RVD position within the CRR. Tal7b EBEs predicted in the promoters using TALE-NT 2.038 are listed below RVDs. C Silencing GhPL1 reduces water-soaked symptoms caused by Xcm TX4 or HM2.2 carrying Tal7b. Assay was done the same as in Fig. 4A. Photos taken at 5 dpi. D Silencing GhPL1 reduces the water-soaked lesion development caused by Xcm TX4 or HM2.2 carrying Tal7b. Assay was done the same as in Fig. 4B. Photos taken at 9 dpi. E GhPL1 is induced by dTAL-PL. Assay was done the same as in Fig. 4G. Data are shown as mean ± s.d. (n = 3) from three independent repeats. Asterisks indicate a significant difference (*p < 0.05) compared to the vector control using a two-tailed Student’s t-test. F, G GhPL1 quantitatively contributes to the water-soaked symptom development, synergistically with GhSWEET14a/b. Xcm HM2.2 carrying dTAL-PL, dTAL-SWEET14a or dTAL-SWEET14b was syringe-inoculated into seven-day-old cotton cotyledons at OD600 = 0.1. Equal proportions of each bacterial inoculum were combined with an empty vector co-inoculum. Xcm HM2.2 carrying Tal7b, co-inoculated with HM2.2 carrying empty vector to maintain the same relative OD600 as each of the others, was included as a control. Photos (F) and water-soaked lesions were analyzed using ImageJ FIJI (G) the same as in (Fig. 1C). Data are shown as the negative grayscale means ± s.e. of lesions relative to Xcm HM2.2 from 9 independent repeats (n = 9) four dpi. Asterisks indicate a significant difference (*p < 0.05, ***p < 0.001, ns = no significance) between treatments using a two-tailed Student’s t-test. The experiments in C, D, F, & G were repeated three times with a similar result. Source data are provided as a Source Data file.
