Fig. 2: CPK1 functions upstream of CNGC5/6/9 for RH growth. | Nature Communications

Fig. 2: CPK1 functions upstream of CNGC5/6/9 for RH growth.

From: CPK1 activates CNGCs through phosphorylation for Ca2+ signaling to promote root hair growth in Arabidopsis

Fig. 2

A set of optical sectioning images of the RHZ of the 4-5 day-old seedlings with a 22.5 µm sectioning step and a sectioning speed of 5 steps per sec were captured under a stereo microscope at room temperature (25 ± 1 °C), and the images were automatically and immediately merged into a 2-D picture for each Petri dish. The merged 2-D pictures were used for RH phenotype analysis. a Typical merged 2-D photos of RHZs of individual seedlings. Scale bars, 0.2 mm in (a). b–d Statistical analyses of RH length (b), branching rates (c), and RH density (d). b–d Numbers of biologically independent roots with approximate 50 RHs per root examined are 11, 13, 13, 13, 10, and 12 for RH length (b), 12, 12, 12, 16, 10, and 11 for RH branching (c), and 16, 14, 9, 13, 11, and 13 for RH density (d), for the lines WT, shrh1, cpk1-1, cpk1 shrh1, CPK1-OE3(shrh1), and CNGC9-OE1(cpk1-1), respectively. Samples with different letters are significantly different with P < 0.05 (one-way ANOVA), and data are presented as means ± SEM in (b–d). Source data are provided as a Source Data file.

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