Fig. 8: The RH phenotypes of shrh1 and cpk1-1 can be rescued by aCNGCs, but not by iCNGCs.

A set of optical sectioning images of the RHZ of the 4-5 day-old seedlings with a 22.5-µm sectioning step and a sectioning speed of 5 steps per sec were captured under a stereo microscope at room temperature (25 ± 1 °C), and each set of images were automatically and immediately merged into a 2-D picture for each Petri dish. The merged 2-D pictures were used for RH phenotype analysis. a–d Typical merged 2-D photos of the RHZ of the seedlings (a), and the statistical analyses of RH length (b), RH branching rates (c), and RH density (d), showing that the RH phenotypes of shrh1 were rescued by the aCNGCs, but not by the iCNGCs. b–d Numbers of biologically independent roots with approximately 50 RHs per root examined are 7, 14, 13, 9, 15, 10, 25, and 12 in (b), 12, 14, 14, 12, 19, 12, 25, and 14 in (c), and 9, 12, 13, 8, 16, 11, 11, and 13 in (d), for the Arabidopsis lines as shown from left to right in each panel. e–h Typical merged 2-D photos of the RHZ of the seedlings (a) and statistical analyses of RH length (f), RH branching rates (g), RH density (h), showing that the RH phenotypes of cpk1-1 were rescued by the aCNGCs, but not by the iCNGCs. e–h Numbers of biologically independent roots with approximate 50 RHs per root examined are 11, 10, 10, 10, 10, 10, 20, and 12 in (f), 12, 8, 11, 10, 10, 10, 13, and 14 in (g), and 8, 10, 10, 10, 10, 10, 12, and 13 in (h), for the Arabidopsis lines as shown from left to right in each panel. Samples with different letters were found to be significantly different with a P < 0.05 (one-way ANOVA), and the data are presented as means ± SEM in (b–d) and (f–h). Source data are provided as a Source Data file.