Fig. 1: Eepd1 is enriched in adipose tissue but reduced in obesity.

a Representative photograph of mice. b Body weight of young and middle-aged mice (n = 7). c Representative photographs of fat pad morphology. d Representative images of H&E staining from iWAT, eWAT, and BAT. Scale bar, 100 μm. e, f Quantification of adipocyte diameters from panel d: iWAT (325 cells from 3 young mice, 153 from 3 middle-aged mice) and eWAT (117 cells from 3 young mice, 111 from 3 middle-aged mice). g Core temperatures of young and middle-aged mice during cold exposure (4 °C for 4 h) (n = 8). h Representative infrared image of young and middle-aged mice exposed to 4 °C for 4 h. i Relative mRNA levels of indicated genes in eWAT of young and middle-aged mice (n = 5). j A Venn diagram depicting the DNA repair genes from the intersection of differentially expressed genes from the RNA-Seq datasets GSE70353 (human) and GSE167264 (mouse), as well as PBmice database with obesity-related genes. k Eepd1 protein expression profile in C57BL/6 J WT mouse tissues as detected by immunoblot. l Immunoblots of Eepd1 in eWAT of young and middle-aged mice. m Immunoblots of Eepd1 in iWAT and eWAT from DIO mice and WT controls of the same age. n Immunoblots of Eepd1 in iWAT and eWAT of 8-week-old male db/db mice and littermate control mice. o Immunoblots of Eepd1 in iWAT and eWAT of 8-week-old male ob/ob mice and WT mice. p Visualization of Eepd1 expression in Single cell sequencing data. q Immunoblots of Eepd1 and Fabp4 in mature adipocytes and SVF from iWAT and eWAT of C57BL/6 J mice. Data presented as mean ± SEM; Significance was assessed by two-way ANOVA (g) or unpaired two-tailed Student’s t test (b, e, f, i). OB, with obesity. iWAT, inguinal white adipose tissue. eWAT, epididymal white adipose tissue. BAT, brown adipose tissue. DIO, diet-induced obesity. SVF, stromal vascular fraction. GAPDH, β-tubulin and β-actin were used as loading controls for Immunoblots. Source data are provided as a Source Data file.