Fig. 2: Eepd1 ablation in adipose tissue enhances middle-age-related obesity progression.
From: Myristoylated Eepd1 Enhances Lipolysis and Thermogenesis through PKA Activation to Combat Obesity
Male Eepd1AKO and littermate Eepd1flox/flox control (Eepd1fl/fl) mice were maintained until 14-month-old to induce middle-aged obesity. a Immunoblots of Eepd1 in adipocytes isolated from iWAT, eWAT and BAT of Eepd1fl/fl and Eepd1AKO mice. β-actin was used as a loading control. b Representative photographs of Eepd1fl/fl and Eepd1AKO mice. c Body weights of Eepd1fl/fl and Eepd1AKO mice (n = 9 for Eepd1fl/fl, n = 8 for Eepd1AKO). d Representative photographs of fat pad morphology. e Representative image of H&E staining from iWAT, eWAT and BAT. Scale bar, 50 μm. f, g Quantification of adipocyte diameters from panel e: iWAT (281 cells from 3 Eepd1fl/fl mice, 119 from 3 Eepd1AKO mice) and eWAT (172 cells from 3 Eepd1fl/fl mice, 110 from 3 Eepd1AKO mice). h Core temperatures of Eepd1fl/fl (n = 9) and Eepd1AKO (n = 5) mice during cold exposure. i Representative infrared images of Eepd1fl/fl and Eepd1AKO mice exposed to 4 °C for 4 h. j Energy expenditure analyzed with ANCOVA using body weight as covariate for Eepd1fl/fl (n = 7) and Eepd1AKO (n = 8) mice. k ANCOVA predicted energy expenditure (EE) at a given body mass of 39 g (n = 7 for Eepd1fl/fl, n = 8 for Eepd1AKO mice). l Plasma insulin concentrations (n = 7 for Eepd1fl/fl, n = 5 for Eepd1AKO mice). m, n GTT and AUC of GTT (n = 6). o, p ITT and AUC of ITT (n = 6 for Eepd1fl/fl, n = 7 for Eepd1AKO). Data presented as mean ± SEM. Significance was assessed by two-way ANOVA (c, h, m, o), two-sided ANCOVA (j), Unpaired two-tailed Student’s t test (k, l, p), or two-sided Mann-Whitney U test (f, g, n). EE, energy expenditure. GTT, glucose tolerance test. ITT, insulin tolerance test. AUC, area under curve. Source data are provided as a Source Data file.
