Fig. 3: Specific ablation of Eepd1 in adipose tissue exacerbates diet-induced obesity development.
From: Myristoylated Eepd1 Enhances Lipolysis and Thermogenesis through PKA Activation to Combat Obesity
a Representative photograph of mice. b Body weights of Eepd1fl/fl (n = 13) and Eepd1AKO (n = 10) mice. c Food intake (n = 4). d Normalized fat mass of Eepd1fl/fl and Eepd1AKO mice via TD-NMR scans (n = 6). e Representative photographs of fat pad morphology. f Representative images of H&E staining of iWAT, eWAT and BAT. Scale bar, 100 μm. g, h Quantification of adipocyte diameters from panel f: iWAT (101 cells from 3 Eepd1fl/fl mice, 53 from 3 Eepd1AKO mice) and eWAT (62 cells from 3 Eepd1fl/fl mice, 61 from 3 Eepd1AKO mice). i Core temperatures of Eepd1fl/fl (n = 11) and Eepd1AKO (n = 7) mice during cold exposure. j Representative infrared images of cold-exposed mice (4 °C, 4 h). k Whole-body energy expenditure under basal conditions during the light and dark phases in Eepd1fl/fl and Eepd1AKO mice (n = 4). l Energy expenditure analyzed with ANCOVA using body weight as covariate for Eepd1fl/fl and Eepd1AKO mice (n = 4). m ANCOVA predicted EE at a given body mass of 36 g (n = 4). n Representative image of H&E staining and IHC of iWAT from Eepd1fl/fl and Eepd1AKO mice after 7 days of cold exposure (8°C). Scale bar, 100 μm. o Immunoblots of Eepd1 in BAT of Eepd1fl/fl and Eepd1AKO mice after 7 days of cold exposure (8°C). β-actin was used as a loading control. p, q qRT‒PCR analysis of indicated genes in iWAT and eWAT of Eepd1fl/fl (n = 13) and Eepd1AKO mice (n = 15). r, s GTT and AUC (n = 12 for Eepd1fl/fl, n = 13 for Eepd1AKO). t, u ITT and AUC (n = 13 for Eepd1fl/fl, n = 16 for Eepd1AKO). Data presented as mean ± SEM. Significance was assessed by two-way ANOVA (b, i, k, r, t), two-sided ANCOVA (l), Unpaired two-tailed Student’s t test (c, d, g, h, m, s), or two-sided Mann-Whitney U test (p, q, u). Source data are provided as a Source Data file.
