Fig. 7: Overexpression of Eepd1 enhances thermogenesis in adipose tissue and ameliorates obesity.
From: Myristoylated Eepd1 Enhances Lipolysis and Thermogenesis through PKA Activation to Combat Obesity
a Workflow for administration of adenovirus in iWAT of DIO mice45. b Representative photographs of fat pad morphology. c Representative H&E staining of dissected tissues. Scale bar, 100 μm. d Quantification of iWAT adipocyte diameters from panel c: 291 cells from 3 Adv-Ctl mice, 349 from 3 Adv-Eepd1 mice. e, f Glycerol and NEFA release in iWAT under basal and ISO-stimulated conditions (n = 8 biological replicates). g Immunoblots of indicated proteins in iWAT injected with Adv-Ctl or Adv-Eepd1. h Body weight gain percentage of DIO mice subcutaneously injected with Adv-Ctl (n = 8) or Adv-Eepd1 (n = 7). i Core temperatures of Adv-Eepd1-injected mice (n = 8). j Energy expenditure under basal conditions during the light and dark phases (n = 6 for Adv-Ctl, n = 7 for Adv-Eepd1). k Energy expenditure analyzed with ANCOVA using body weight as covariate (n = 6 for Adv-Ctl, n = 7 for Adv-Eepd1). l ANCOVA predicted EE at a given body mass of 34 g (n = 6 for Adv-Ctl, n = 7 for Adv-Eepd1). m qRT‒PCR analysis of indicated genes in iWAT (n = 8 for Adv-Ctl, n = 6 for Adv-Eepd1). n Body weights of mice injected with AAV-Ctl or AAV-Eepd1 (n = 7). o, p qRT‒PCR analysis of indicated genes in iWAT (n = 7). q Immunoblots of Eepd1 in iWAT treated with Vehicle or Retigabine dihydrochloride. r Body weight of mice treated with Vehicle or Retigabine dihydrochloride for indicated time (n = 8). s Representative photographs of fat pad morphology. t Representative H&E staining of dissected tissues. Scale bar, 100 μm. u Immunoblots of indicated proteins in iWAT treated with Vehicle or Retigabine dihydrochloride. Data presented as mean ± SEM. Significance was assessed by two-way ANOVA (i, j, n, r), two-sided ANCOVA (k), unpaired two-tailed Student’s t test (e, f, h, l), or two-sided Mann-Whitney U test (d, m, o, p). Adv-Ctl, pAdEasy-EF1-MCS-3xFlag-CMV-EGFP; Adv-Eepd1, pAdEasy-EF1-MCS-Eepd1-3xFlag-CMV-EGFP; AAV-Ctl, pAAV2/9-CMV-3xFlag-P2A-mNeonGreen-tWPA; AAV-Eepd1, pAAV2/9-CMV-Eepd1-3xFlag-P2A-mNeonGreen-tWPA. Source data are provided as a Source Data file.
