Fig. 8: EEPD1 expression in human adipose tissue and its impact on lipolytic function.
From: Myristoylated Eepd1 Enhances Lipolysis and Thermogenesis through PKA Activation to Combat Obesity
a Association analysis of sequence variants with WHRadjBMI. The common SNP rs17275193 that significantly associated with WHRadjBMI in humans was indicated by a purple dot. b Single-cell RNA sequencing analysis of EEPD1 expression in VAT of human with BMI < 30 and BMI > 30. c Immunoblots of EEPD1 in SVF and adipocytes isolated from human adipose tissue. GAPDH was used as a loading control. d Violin plot showing single-cell RNA sequencing analysis of EEPD1 expression in human visceral adipose tissues. e Linear regression analysis of BMI and EEPD1 mRNA expression in human subcutaneous adipose tissues (n = 48). f Linear regression analysis of BMI and EEPD1 mRNA expression in human visceral adipose tissues (n = 46). g Immunoblots of indicated proteins in primary human adipocytes treated with NC or siEEPD1 for 48 h. β-actin was used as a loading control. h, i Glycerol and NEFA release assay in primary human adipocytes treated with NC or siEEPD1 for 48 h (n = 4 biological replicates). j qRT‒PCR analysis of indicated genes in human adipocytes transfected with siRNAs for 48 h (n = 4 biological replicates). k OCR in primary human adipocytes measured by Seahorse assay (n = 6 biological replicates). l Immunoblots of indicated proteins in primary human adipocytes transfected with mock or EEPD1-Flag vectors for 48 h. β-actin was used as a loading control. m, n Glycerol and NEFA release assay in primary human adipocytes transfected with mock or EEPD1-Flag vectors for 48 h (n = 4 biological replicates). o qRT‒PCR analysis of indicated genes in human adipocytes transfected with mock or EEPD1-Flag vectors for 48 h (n = 4 biological replicates). p OCR in primary human adipocytes measured by Seahorse assay (n = 7 biological replicates). The data are presented as the mean ± SEM. Significance was assessed by Pearson correlation analysis (e, f), unpaired two-tailed Student’s t test (d, n, p), one-way ANOVA (h, i, j, k) or two-sided Mann-Whitney U test (m, o). Source data are provided as a Source Data file.
