Fig. 6: Altered centromere positioning enables telomeres to recruit bouquet proteins Bqt1 and Bqt2, triggering telocentrosome formation. | Nature Communications

Fig. 6: Altered centromere positioning enables telomeres to recruit bouquet proteins Bqt1 and Bqt2, triggering telocentrosome formation.

From: Centromere positioning orchestrates telomere bouquet formation and the initiation of meiotic differentiation

Fig. 6

a-b Bqt1 and Bqt2 were ectopically expressed in haploid mitotic cells and localization of Taz1-mCherry, Bqt1-GFP, and Ppc89-CFP was examined along with key telocentrosome components. Scale bars: 2 μm. c Quantification of phenotypes observed in (a-b), 150 cells per genotype from three independent experiments were analyzed. P value derived from two-sided Fisher’s exact test is shown. d sad1-2 csi1Δ cell where the telomere is stably associated with the SPB is shown. e Quantification of the phenotype observed in (d); 150 cells per genotype from three independent experiments were scored and mean value +/- SEM shown, p value from two-tailed Student’s t-test analysis. f-g SGA experiment as in Fig. 1d. Colony growth rate was analyzed using hierarchical clustering with Euclidean distance and the complete linkage method to identify gene clusters (two-sided p value < 0.001).

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