Fig. 7: The outer kinetochore disassembles upon bouquet formation in diploid meiotic prophase. | Nature Communications

Fig. 7: The outer kinetochore disassembles upon bouquet formation in diploid meiotic prophase.

From: Centromere positioning orchestrates telomere bouquet formation and the initiation of meiotic differentiation

Fig. 7

a-b Frames from time-lapse experiment of meiotic diploid cells expressing endogenously tagged Ndc80-GFP and Taz1-mCherry. c Quantification of Ndc80-GFP intensity throughout the experiment as depicted in panels (a) and (b), with each color representing the behavior of 9 independent cells. d Meiotic diploid cells were analyzed as in (a-b), but with one copy of Sad1 endogenously tagged with GBP. e-f Exponentially growing diploid cells were induced to undergo meiosis. The percentage of cells with the indicated number of nuclei per cell is shown for 200 cells at each time point across three independent experiments. g Frames from a time-lapse experiment of meiotic diploid cells with endogenously tagged Cdc2-GFP and Taz1-mCherry. Yellow arrows indicate early and late co-localization between telomeres and Cdc2. h Quantification of Cdc2-GFP intensity throughout the experiment as depicted in panel (g), with each color representing the behavior of 7 independent cells. Scale bars, 5 μm.

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