Fig. 4: K. pneumoniae dynamics in secondary organs is influenced by the mode of dissemination.
From: Patterns of Klebsiella pneumoniae bacteremic dissemination from the lung
Wild-type mice were infected with a KPPR1-STAMPR (purple circles or pink hexagons), gmhB-STAMPR (blue triangles), or tatC-STAMPR (green triangles) K. pneumoniae barcoded library. Nox2-/- (gold diamonds) or Ccr2-/- (yellow squares) mice were infected with the KPPR1-STAMPR barcoded library. For all, an inoculum of 1 × 106 CFU/mouse was administered in a model of pneumonia that progresses to bacteremia and tissues were harvested 24-hours post infection. Wild-type mice infected with the KPPR1 library were divided into groups demonstrating metastatic (KPPR1-met, purple circles) or direct (KPPR1-dir, pink hexagons) dissemination as defined in Fig. 3. The bacterial burden at the time of harvest in the (A) lung, (B) spleen, (C) liver, or (D) blood for each infection group is displayed as log10 CFU. The founding population in each tissue was estimated by the STAMPR pipeline for the (E) lung, (F) spleen, (G) liver, or (H) blood for each group and displayed as log10 Ns. A measure of total CFU per founder is represented by log10(CFU/Ns) for the (I) lung, (J) spleen, (K) liver, or (L) blood for each group. In (A–L), bars represent the mean and points represent individual animals. For all, n = 5–9 mice in at least two independent trials; specifically, KPPR1-met n = 8, KPPR1-dir n = 9, gmhB n = 9, tatC n = 5, Nox2-/- n = 5, Ccr2-/- n = 8. Comparisons between the KPPR1-met group and each other group were assessed using an ordinary one-way ANOVA with Dunnet’s multiple comparisons correction and indicated by *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Comparisons between the KPPR1-dir group and each other group were also assessed using an ordinary one-way ANOVA with Dunnet’s multiple comparisons correction and indicated by #p < 0.05, ###p < 0.001, ####p < 0.0001. For clarity, only p-values < 0.1 are displayed on in A–L. Source data for each panel are provided within the Source Data file, including the every p-value measured for the one-way ANOVA comparisons.
