Fig. 5: Dissemination and bacteremia kinetics are influenced by bacterial and host factors.

Wild-type mice were infected with a (A, B) gmhB-STAMPR (blue circles) or (C, D) tatC-STAMPR (green circles) K. pneumoniae barcoded libraries, while (E, F) Nox2^-/- (gold circles) or (G, H) Ccr2^-/- (yellow circles) mice were infected with the wild-type KPPR1-STAMPR library. In all experiments, an inoculum of 1×10⁶ CFU/mouse was administered in a model of pneumonia that progresses to bacteremia and tissues were harvested 24-hours post-infection. In (A–H), genetic distance (GD) and number of barcodes shared (FRD) between the lung:spleen (A, C, E, G) or lung:liver (B, D, F, H) are displayed with indications for whether dissemination is classified as metastatic (closed symbols) or direct (open symbols), further defined in Supplementary Fig. 3. I The lung log₁₀(N_s/N_b) is displayed for all groups. In (J–N), wild-type mice infected with the KPPR1 library were divided into groups demonstrating metastatic (KPPR1-met) or direct (KPPR1-dir) dissemination. The GD between the lung:spleen (J), FRD between the lung:spleen (K), GD between the lung:liver (L), FRD between the lung:liver (M), and the GD between the spleen:liver (N) is displayed for all infected groups. In (I–N), data is represented as a box plot with points representing individual animals, whisker end points indicating the minimum and maximum observed values, box boundaries representing the 25th and 75th percentile, and the middle line representing the median value. KPPR1-met is displayed as purple circles, KPPR1-dir is displayed as pink open circles, gmhB-STAMPR is displayed as blue triangles, tatC-STAMPR is displayed as green triangles, Nox2^-/- mice are displayed as gold triangles, and Ccr2^-/- mice are displayed as yellow squares. For all, n = 5–9 mice in at least two independent trials; specifically, KPPR1-met n = 8, KPPR1-dir n = 9, gmhB n = 9, tatC n = 5, Nox2^-/- n = 5, Ccr2^-/- n = 8. Comparisons between the KPPR1-met group and each other group were assessed using an ordinary one-way ANOVA with Dunnet’s multiple comparisons correction, p-values for each comparison are indicated in (I–N). Source data for each panel are provided within the Source Data file.