Fig. 2: Characterization of polypeptide copolymers and hydrogel properties.

A Plot of the intensity ratios (I₃₃₆/I₃₃₃) to log₁₀C in CMC test. B CD spectra of three copolymers in aqueous solution. The experiments were repeated two times and representative data from one of the experiments are shown. C Sol-gel phase diagrams of copolymer solutions (n = 4 experimental replicates in each group). D Rheological properties of copolymer solutions of 6.0 wt%. Release behavior of CpG-ODN (E) and OVA-Cy5 (F) in vitro (n = 3 experimental replicates in each group). The mass remaining of degradation behavior of hydrogels in vitro (G) and in vivo (H) (n = 3 experimental replicates in each group). I Representative image of hydrogels at 37 °C (top) and the porous structure of hydrogels photographed by cryo-SEM (bottom), scale bar: 5 μm. The experiments were repeated at least two times and representative data from one of the experiments are shown. J Hematoxylin-eosin (H&E) stain of hydrogels and surrounded tissues at 1 and 2 weeks in vivo, scale bar: 1 mm. Data are presented as the mean ± SEM. Source data are provided as a Source Data file.