Fig. 5: The anti-tumor effect in prophylactic treatment.

A Schematic illustration of the prophylactic treatment. B Individual tumor growth kinetics in each group (n = 8 mice in each group). CR was defined as complete disappearance of tumor on day 24. The experiments were repeated three times and representative data from one of the experiments are shown. C Average tumor growth curve (n = 8 mice in each group). The experiments were repeated three times and representative data from one of the experiments are shown. D The tumor weights of different groups on day 24 (n = 8 mice in each group). E Tumor images on day 24 in the prophylactic experiment (n = 8 mice in each group). F The SIINFEKL-H2Kb positive DCs in LNs (n = 6 mice in each group). The IFN-γ positive cells within CD8⁺ T cells (G) and CD4⁺ T cells (H) in peripheral blood (n = 6 mice in each group). The proportion of central memory T cells (CD44⁺CD62L⁺) within CD4⁺ T cells (I) and CD8⁺ T cells (J) in spleens (n = 6 mice in each group). K Representative flow cytometry images of memory T cells. Data are presented as the mean ± SEM. G1: PBS, G2: Free Vac, G3: Alum Vac, G4: D-Gel Vac and G5: L-Gel Vac. Statistical significance was analyzed by one-way ANOVA using LSD posttest (C, D) or one-way ANOVA using Tukey’s posttest (F–J). Source data are provided as a Source Data file.